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NASC Stock Detail Page

NASC ID: N585869

Name: SALK_085869

ABRC stock number: SALK_085869

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

July 2002 TAIR gene annotation - At5g13500. T6I14.30 R300-3'. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_085869.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G13510.1 (at chr 5 pos 4340941 (W/4340878-4340941) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 69, R 67, C 67 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO428247.
SALKseq_085869.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G20830.1=P355 and AT3G20840.1=P355 (at chr 3 pos 7293786 (C/7293786-7293849) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 69, R 67, C 67 N 68. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO424932.

Donation Date: 2002-01-07

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_085869

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_085869C.

Status Price (£)
Available £10.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
EMBL accession:

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.