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NASC ID: N586036

Name: SALK_086036

ABRC stock number: SALK_086036

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

July 2002 TAIR gene annotation - At5g56170. MDA7.23 Exon. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_086036.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G56170.1 (at chr 5 pos 22737013 (W/22736952-22737013) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 74, R 77, C 71 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO424964.

SALKseq_086036.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G31530.1 and AT1G31540.2=UTR3 (at chr 1 pos 11286357 (C/11286357-11286402) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 52-78 bps onto the T-DNA. Pools P 74, R 77, C 71 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO438204.

SALKseq_086036.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G26180.1 (at chr 1 pos 9054787 (W/9054722-9054787) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 78-95 bps onto the T-DNA. Pools P 74, R 77, C 71 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO418331.

SALKseq_086036.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G04997.1 and AT3G05000.1=UTR3 (at chr 3 pos 1386894 (W/1386837-1386894) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 74, R 77, C 71 N 79. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO421647.

Donation Date: 2002-01-07

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_086036

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_086036C.

Status Price (£)
Available £10.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
EMBL accession:

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.