NASC Stock Detail Page
NASC ID: N586228
Name: SALK_086228
ABRC stock number: SALK_086228
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_086228.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G27240.1 and AT2G27250.1=UTR3 (at chr 2 pos 11664479 (W/11664420-11664479) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 70, R 61, C 69 N 70. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO428314. SALKseq_086228.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G38600.1 (at chr 4 pos 18043371 (W/18043316-18043371) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 70, R 61, C 69 N 70. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO431630. SALKseq_086228.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G37360.1 (at chr 5 pos 14808234 (C/14808234-14808302) on the TAIR10). The sequences' 1-69 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 70, R 61, C 69 N 70. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO434937.
Donation Date: 2003-01-30
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_086228
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N664735 (SALK_086228C).
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.