NASC

NASC ID: N586257

Name: SALK_086257

ABRC stock number: SALK_086257

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_086257.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G57270.1 (at chr 3 pos 21193398 (W/21193339-21193398) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 68, R 70, C 64 N 69. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO428320.
SALKseq_086257.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G44740.1=P355 and AT2G44745.1=UTR3 (at chr 2 pos 18444232 (W/18444173-18444232) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 68, R 70, C 64 N 69. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO425006.

Donation Date: 2003-01-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_086257

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_086257C.

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G44740 AT2G44745 At3g57270

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.