NASC

NASC ID: N588536

Name: SALK_088536

ABRC stock number: SALK_088536

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

August 2002 TAIR gene annotation - K13E13.9 L300-5', At3g18980. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_088536.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G18980.1 (at chr 3 pos 6546218 (W/6546141-6546218) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 76, R 78, C 79 N 71. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO428750.

SALKseq_088536.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G59930.1 and AT1G59940.1=UTR3 (at chr 1 pos 22065455 (C/22065455-22065506) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 37-77 bps onto the T-DNA. Pools P 76, R 78, C 79 N 71. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO425436.

SALKseq_088536.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G60880.2=P355 and AT5G60890.1=P355 (at chr 5 pos 24491297 (W/24491260-24491297) on the TAIR10). The sequences' 1-38 bps mapped on the genome, 44-70 bps onto the T-DNA. Pools P 76, R 78, C 79 N 71. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO432066.

Donation Date: 2002-01-09

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_088536

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_088536C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G59930 AT1G59940 AT5G60880 AT5G60890 At3g18980

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.