Home | About NASC | Address/Staff
Ask a question | Help

NASC

Please note that due to the global coronavirus epidemic orders placed with NASC may take longer than usual to reach you

NASC Stock Detail Page

NASC ID: N589247

Name: SALK_089247

ABRC stock number: SALK_089247

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.August 2002 TAIR gene annotation - MOE17.14 Exon, At3g20865. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms:

SALKseq_089247.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G20865.1 (at chr 3 pos 7308848 (C/7308848-7308910) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 2, R 9, C 6 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO425565.
SALKseq_089247.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G67340.1 (at chr 1 pos 25230158 (W/25230091-25230158) on the TAIR10). The sequences' 1-67 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 2, R 9, C 6 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO422248.

Donation Date: 2002-01-09

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_089247

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N664744 (SALK_089247C).

Status Price (£)
Available £10.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
EMBL accession:

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.