NASC Stock Detail Page
NASC ID: N590900
Name: SALK_090900
ABRC stock number: SALK_090900
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. August 2002 TAIR gene annotation - T30L20.5 L300-5', At2g22790. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2. Associated Polymorphisms: SALKseq_090900.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G22780.1=P355 and AT2G22790.1 (at chr 2 pos 9695845 (C/9695845-9695908) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 79-100 bps onto the T-DNA. Pools P 80, R 72, C 75 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO419199. SALKseq_090900.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G19300.1=P355 and AT1G19310.1=UTR3 (at chr 1 pos 6675325 (C/6675325-6675380) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 80, R 72, C 75 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO439071. SALKseq_090900.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G28910.2=P355 and AT5G28913.1 (at chr 5 pos 10933624 (C/10933624-10933658) on the TAIR10). The sequences' 1-35 bps mapped on the genome, 59-78 bps onto the T-DNA. Pools P 80, R 72, C 75 N 74. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO422515.
Donation Date: 2002-01-09
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_090900
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_090900C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.