NASC

NASC ID: N592571

Name: SALK_092571

ABRC stock number: SALK_092571

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

December 2002 TAIR gene annotation - At5g25350 F18G18.90 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_092571.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G25350.1 (at chr 5 pos 8795096 (C/8795096-8795154) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 79, R 78, C 75 N 71. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO439267.

SALKseq_092571.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G28760.1 (at chr 2 pos 12337946 (W/12337885-12337946) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 79, R 78, C 75 N 71. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO432656.

SALKseq_092571.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G25340.1 (at chr 5 pos 8792995 (W/8792947-8792995) on the TAIR10). The sequences' 1-49 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 79, R 78, C 75 N 71. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO435965.

Donation Date: 2003-01-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_092571

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_092571C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G28760 AT5G25340 At5g25350

EMBL accession:

BZ596433

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.