NASC Stock Detail Page
NASC ID: N610931
Name: SALK_110931
ABRC stock number: SALK_110931
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_110931.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G40076 and AT1G40390.1 (around chr 1 pos 15428897 on the TAIR10). The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO415990. SALKseq_110931.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G59980.1=UTR3 and AT1G59990.1=UTR3 (at chr 1 pos 22088387 (W/22088346-22088387) on the TAIR10). The sequences' 1-42 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 20, R 18, C 13 N 19. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO380016. SALKseq_110931.3 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G06922 and AT2G06925.1=UTR3 (at chr 2 pos 2841248 (W/2841170-2841248) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 20, R 18, C 13 N 19. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO383361.
Donation Date: 2003-01-31
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_110931
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_110931C.
Status | Price (£) |
---|---|
Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
---|---|---|---|
|
|
|
Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.