NASC

NASC ID: N628671

Name: SALK_128671

ABRC stock number: SALK_128671

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

January 2003 TAIR gene annotation - At1g24230 F3I6.16 Intron Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_128671.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G24230.1 (at chr 1 pos 8584339 (C/8584339-8584406) on the TAIR10). The sequences' 1-68 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 61, R 65, C 67 N 70. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO387119.

SALKseq_128671.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G39260.1 (at chr 2 pos 16399571 (C/16399571-16399633) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 56-95 bps onto the T-DNA. Pools P 61, R 65, C 67 N 70. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO390465.

SALKseq_128671.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G24710.1 (at chr 5 pos 8465589 (W/8465536-8465589) on the TAIR10). The sequences' 2-55 bps mapped on the genome, 55-79 bps onto the T-DNA. Pools P 61, R 65, C 67 N 70. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO393819.

Donation Date: 2003-01-31

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_128671

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_128671C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G39260 AT5G24710 At1g24230

EMBL accession:

BZ356254

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.