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NASC Stock Detail Page

NASC ID: N635536

Name: SALK_135536

ABRC stock number: SALK_135536

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_135536.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G13245.1 (at chr 1 pos 4526260 (W/4526205-4526260) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 69, R 66, C 63 N 65. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO407491.

SALKseq_135536.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G35840.1 (at chr 2 pos 15055209 (W/15055172-15055209) on the TAIR10). The sequences' 1-38 bps mapped on the genome, 53-79 bps onto the T-DNA. Pools P 69, R 66, C 63 N 65. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO410836.

SALKseq_135536.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G04721.1 and AT3G04730.1=UTR3 (at chr 3 pos 1288093 (W/1288015-1288093) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 69, R 66, C 63 N 65. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO414187.

Donation Date: 2003-03-11

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_135536

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_135536C.

Status Price (£)
Available £7.50

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.