NASC

NASC ID: N636671

Name: SALK_136671

ABRC stock number: SALK_136671

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_136671.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G14330.1 (at chr 5 pos 4620347 (C/4620347-4620401) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 78, R 71, C 78 N 77. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO390828.

SALKseq_136671.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G65420.1=P355 and AT1G65430.1=UTR3 (at chr 1 pos 24299851 (W/24299793-24299851) on the TAIR10). The sequences' 1-59 bps mapped on the genome, 54-78 bps onto the T-DNA. Pools P 78, R 71, C 78 N 77. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO384137.

SALKseq_136671.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G33150.1=P355 and AT2G33160.1 (at chr 2 pos 14052533 (W/14052477-14052533) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 78, R 71, C 78 N 77. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO387487.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_136671

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_136671C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G65420 AT1G65430 AT2G33150 AT2G33160 At5g14330

EMBL accession:

CC796694

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.