NASC

NASC ID: N637187

Name: SALK_137187

ABRC stock number: SALK_137187

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

February 2003 TAIR gene annotation - At1g08260 T23G18. 21 Intron T23G18. 21 Intron. Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

Associated Polymorphisms:

SALKseq_137187.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intron of AT1G08260.1 (at chr 1 pos 2599493 (C/2599493-2599550) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 63, R 64, C 67 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO347352.

SALKseq_137187.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G05620.1 (at chr 1 pos 1680958 (C/1680958-1681025) on the TAIR10). The sequences' 1-68 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 63, R 64, C 67 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO344005.

SALKseq_137187.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G18350.1 (at chr 1 pos 6316612 (W/6316577-6316612) on the TAIR10). The sequences' 1-35 bps mapped on the genome, 42-67 bps onto the T-DNA. Pools P 63, R 64, C 67 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO350697.

SALKseq_137187.2 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G57070.1 (at chr 3 pos 21123987 (C/21123987-21124023) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 63, R 64, C 67 N 63. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO354041.

Donation Date: 2003-03-11

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_137187

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_137187C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G05620 AT1G18350 AT3G57070 At1g08260

EMBL accession:

BZ766268

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.