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NASC Stock Detail Page

NASC ID: N638830

Name: SALK_138830

ABRC stock number: SALK_138830

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_138830.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT2G31190.1 (at chr 2 pos 13291397 (W/13291356-13291397) on the TAIR10). The sequences' 1-42 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 7, R 3, C 3 N 9. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO357452.

SALKseq_138830.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G04040.1 (at chr 4 pos 1941397 (C/1941397-1941457) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 7, R 3, C 3 N 9. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO360801.

SALKseq_138830.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the UTR 3' of AT5G14610.1 (at chr 5 pos 4714783 (W/4714734-4714783) on the TAIR10). The sequences' 1-50 bps mapped on the genome, 54-79 bps onto the T-DNA. Pools P 7, R 3, C 3 N 9. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO364155.

Donation Date: 2003-03-11

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_138830

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_138830C.

Status Price (£)
Available £10.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
EMBL accession:

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2.

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.