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NASC Stock Detail Page

NASC ID: N6947

Name: til1-4 / til1-4

ABRC stock number: CS6947

Description: A line homozygous for the shoot apical meristem-expressed PSTM:GUS reporter (the promoter of the SHOOT MERISTEMLESS gene fused to beta-glucuronidase) was mutagenized with ethyl methanesulfonate and screened for embryo-defective mutants.

Donation Date: 2006-09-01

Donated by:  University of Wisconsin, Madison Kathy Barton
Franklin and Marshall College Pablo Jenik

Donor Number: til1-4 / til1-4

Part of : Homozygous lines

Stock type: individual line

Material type: seed

Comment: gene name:TILTED 1, recessive mutation

Status Price (£)
Available £7.50

Germplasm Info

Seed type: Mutant

Mutagen: ethylmethane sulfonate

Background: Col

Segregation status: homozygous

Pedigree: Backcrossed 3 times to Col.

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
  • til1-4
  • til1

Phenotype

Weak allele. Embryos have altered placement of the root pole, slow embryonic development, longer cell cycles (~35%) and larger cells. Abnormal division of the hypophysis in the developing root of the embryo, generating a lens cell that is abnormal in shape or abnormally positioned; asymmetric development of the provasculature, this ultimately leads to an abnormal root pole which is displaced laterally causing the root-shoot axis to be at an angle to the long axis of the suspensor, giving the embryo a tilted appearance. Embryos are viable, have larger cells and nuclei than wild-type embryos but the same DNA content, mutant embryos are larger than wild-type embryos at the same developmental stage. Plants have slow growing roots, slightly delayed flowering, altered floral phyllotaxis, reduced number of ovules, abnormally developing ovules, and reduced fertility.