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NASC Stock Detail Page

NASC ID: N6952

Name: G protein-coupled receptor

ABRC stock number: CS6952

Description: Knockout line harboring a T-DNA insertion in intron 2 (at 506 bp) of the GCR1 gene, was found in a PCR screen of a collection of 72,960 T-DNA-inserted Arabidopsis lines (Arabidopsis Knockout Facility, University of Wisconsin, BASTA population) using a GCR1-specific primer and a T-DNA left border-specific primer; homozygous mutant line for At1g 48270 (GCR1 gene).

Donation Date: 2006-10-16

Donated by:  Pennsylvania State University Sally Assmann
University of Missouri John Walker
Franklin and Marshall College Pablo Jenik

Donor Number: gcr1-3

Part of : Homozygous lines

Stock type: individual line

Material type: seed

Comment: recessive

Status Price (£)
Available £7.50

Germplasm Info

Mutagen: T-DNA Insertion

Background: Ws-2 ( N2360)

Segregation status: homozygous

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
  • gcr1-3

Phenotype

Plants exhibit hypersensitivity to abscisic acid (ABA) in assays of root growth, gene regulation, and stomatal response. Root growth of seedlings is markedly more sensitive to inhibition by ABA compared with wild-type. Plants have enhanced drought tolerance and lower rates of water loss (lower transpiration rates). Plants are hypersensitive to ABA and S1P regulation of stomatal responses: guard cells are hypersensitive to both inhibition of opening of preclosed stomata and promotion of closure of preopened stomata by ABA; plants respond to the lipid metabolite, sphingosine-1-phosphate (S1P), in an identical fashion as they do to ABA: guard cells are hypersensitive to S1P-induced inhibition of stomatal opening and S1P-induced promotion of stomatal closure compared with the wild-type.

References

Pandey, S. & Assmann, S.M. 2004. The Arabidopsis putative G protein-coupled receptor GCR1 interacts with the G protein alpha subunit GPA1 and regulates abscisic acid signaling. The Plant Cell 16(6):1616-32.PubMed ID: 15155892.