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NASC Stock Detail Page

NASC ID: N809354

Name: SAIL_199_E01

ABRC stock number: CS809354

Description: T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pCSA110, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; homozygous for qrt (quartet, pollen tetrad defect) mutation; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection.

Associated Polymorphisms:

SAILseq_199_E01.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G54740.1 (at chr 3 pos 20262442 (W/20262380-20262442) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 74-95 bps onto the T-DNA. Pools P 18, R 15, C 12 N 17. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO336056.

SAIL_199_E01

Donation Date: 2004-06-01

Donated by:  Syngenta AG Alberto Martinez

Donor Number: SAIL_199_E01

Part of : SAIL lines from Syngenta

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SAIL_199_E01.

Status Price (£)
Available £10.00

Germplasm Info

Background: Col-3( N8846)

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
EMBL accession:

References

McElver, J. et al. 2001. Insertional mutagenesis of genes required for seed development in Arabidopsis thaliana. Genetics 159(4):1751-63.PubMed ID: 11779812.