Home | About NASC | Address/Staff
Ask a question | Help

NASC

From January 1st, 2021, seed orders from EU customers will require a phytosanitary certificate. Please click for further information

NASC Stock Detail Page

NASC ID: N810254

Name: SAIL_221_H01

ABRC stock number: CS810254

Description: T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pCSA110, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; homozygous for qrt (quartet, pollen tetrad defect) mutation; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection.

Associated Polymorphisms:

SAILseq_221_H01.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G10580.1 (at chr 1 pos 3493920 (W/3493826-3493920) on the TAIR10). The sequences' 1-95 bps mapped on the genome. Pools P 19, R 11, C 14 N 20. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO334084.

SAILseq_221_H01.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G03770.2-P355 and AT3G03773.1-P355 (at chr 3 pos 950027 (W/949975-950027) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 66-92 bps onto the T-DNA. Pools P 19, R 11, C 14 N 20. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO336076.

SAILseq_221_H01.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G05310.1 and AT4G05320.1-P355 (at chr 4 pos 2717734 (C/2717734-2717805) on the TAIR10). The sequences' 2-73 bps mapped on the genome, 72-95 bps onto the T-DNA. Pools P 19, R 11, C 14 N 20. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO326582.

SAIL_221_H01 .

Donation Date: 2004-06-01

Donated by:  Syngenta AG Alberto Martinez

Donor Number: SAIL_221_H01

Part of : SAIL lines from Syngenta

Stock type: individual line

Material type: seed

Status Price (£)
Available £10.00

Germplasm Info

Background: Col-3( N8846)

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene: Allele: Locus: AGI code:
EMBL accession:

References

McElver, J. et al. 2001. Insertional mutagenesis of genes required for seed development in Arabidopsis thaliana. Genetics 159(4):1751-63.PubMed ID: 11779812.