NASC

NASC ID: N814962

Name: SAIL_323_E01

ABRC stock number: CS814962

Description: T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pCSA110, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; homozygous for qrt (quartet, pollen tetrad defect) mutation; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection.

Associated Polymorphisms:

SAILseq_323_E01.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G63170.1 and AT5G63180.1-UTR3 (at chr 5 pos 25340590 (W/25340517-25340590) on the TAIR10). The sequences' 1-74 bps mapped on the genome. Pools P 22, R 23, C 23 N 23. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO336272.

SAILseq_323_E01.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G24930.1 (at chr 5 pos 8589020 (W/8588956-8589020) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 66-93 bps onto the T-DNA. Pools P 22, R 23, C 23 N 23. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO334280.

SAIL_323_E01