NASC

NASC ID: N818500

Name: SAIL_400_E05

ABRC stock number: CS818500

Description: T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pCSA110, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; homozygous for qrt (quartet, pollen tetrad defect) mutation; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection.

Associated Polymorphisms:

SAILseq_400_E05.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G18215.1 (at chr 3 pos 6242187 (W/6242128-6242187) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 60-95 bps onto the T-DNA. Pools P 24, R 23, C 21 N 24. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO336499.

SAILseq_400_E05.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G48540.1 (at chr 3 pos 17990293 (C/17990293-17990361) on the TAIR10). The sequences' 1-69 bps mapped on the genome, 70-95 bps onto the T-DNA. Pools P 24, R 23, C 21 N 24. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO329139.

SAIL_400_E05