NASC

NASC ID: N850306

Name: WiscDsLox289_292I19

ABRC stock number: CS850306

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

WiscDsLox289_292I19 .

Wiscseq_DsLox289_292I19.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G13150.1 and AT5G13160.1-P355 (chr 5 pos 4175258 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG797567.

Wiscseq_DsLox289_292I19.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G36030.1-P355 and AT4G36040.1-UTR3 (chr 4 pos 17048105 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG797566.

Wiscseq_DsLox289_292I19.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G11550.1 (at chr 5 pos 3709277 (C/3709277-3709360) on the TAIR10). The sequences' 1-84 bps mapped on the genome, 86-100 bps onto the T-DNA. Pools P 1, R 2, C 4 N 8. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO453004.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox289_292I19

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	WiscDsLox289_292I19	WiscDsLox289_292I19	AT4G36030 AT4G36040 AT5G11550 AT5G13160 At5g13150