NASC Stock Detail Page
NASC ID: N850590
Name: WiscDsLox293-296invH3
ABRC stock number: CS850590
Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line. Associated Polymorphisms: Wiscseq_DsLox293-296invH3.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G09153.1 and AT4G09160.1-P355 (at chr 4 pos 5835654 (C/5835654-5835735) on the TAIR10). The sequences' 1-82 bps mapped on the genome, 80-100 bps onto the T-DNA. Pools P 1, R 5, C 1 N 6. The insertion site is at the 3' end of the sequence mapping. Genbank ID: KO452694.
Donation Date: 2006-09-04
| Donated by: | University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman |
Donor Number: WiscDsLox293-296invH3
Part of : Wisconsin DsLox T-DNA lines
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: T-DNA Insertion
Background: Col-2
Transformation method: Agrobacterium
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
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