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NASC Stock Detail Page

NASC ID: N850590

Name: WiscDsLox293-296invH3

ABRC stock number: CS850590

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

WiscDsLox293-296invH3 .

Wiscseq_DsLox293-296invH3.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G09153.1 and AT4G09160.1-P355 (at chr 4 pos 5835654 (C/5835654-5835735) on the TAIR10). The sequences' 1-82 bps mapped on the genome, 80-100 bps onto the T-DNA. Pools P 1, R 5, C 1 N 6. The insertion site is at the 3' end of the sequence mapping. Genbank ID: KO452694.

Donation Date: 2006-09-04

Donated by:  University of Wisconsin, Madison Patrick Krysan
University of Wisconsin, Madison Richard Amasino
University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox293-296invH3

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status Price (£)
Available £10.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
  • WiscDsLox293-296invH3
  • WiscDsLox293