NASC

NASC ID: N852417

Name: WiscDsLox353H07

ABRC stock number: CS852417

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

WiscDsLox353H07 .

Wiscseq_DsLox353H07.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G44740.1 (at chr 2 pos 18443635 (C/18443635-18443710) on the TAIR10). The sequences' 1-76 bps mapped on the genome, 83-100 bps onto the T-DNA. Pools P 3, R 2, C 4 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank ID: KO453038.

Wiscseq_DsLox353H07.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G44740.1 and AT1G44750.1-P355 (chr 1 pos 16889301 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG800472.

Wiscseq_DsLox353H07.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G07960.1 (at chr 3 pos 2534809 (C/2534809-2534866) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 82-100 bps onto the T-DNA. Pools P 3, R 2, C 4 N 7. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO453337.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox353H07

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	WiscDsLox353H07	WiscDsLox353H07	AT1G44740 AT1G44750 AT3G07960 At2g44740