NASC

NASC ID: N852871

Name: WiscDsLox363A01

ABRC stock number: CS852871

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

WiscDsLox363A01 .

Wiscseq_DsLox363A01.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G75620.1 (chr 1 pos 28395513 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank ID: KG800992.

Wiscseq_DsLox363A01.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G70770.1-P355 and AT1G70780.1-UTR3 (chr 1 pos 26692479 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG800991.

Wiscseq_DsLox363A01.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G28830.1-P355 and AT2G28840.1-P355 (chr 2 pos 12377908 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG800993.

Donation Date: 2004-12-01

Donated by:

University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox363A01

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
	WiscDsLox363A01	WiscDsLox363A01	AT1G70770 AT1G70780 AT2G28830 AT2G28840 At1g75620

EMBL accession:

CW794450