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NASC Stock Detail Page

NASC ID: N855860

Name: WiscDsLox439B09

ABRC stock number: CS855860

Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

Associated Polymorphisms:

WiscDsLox439B09 .

Wiscseq_DsLox439B09.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G65070.1 (at chr 5 pos 25992149 (C/25992149-25992196) on the TAIR10). The sequences' 1-46 bps mapped on the genome, 45-100 bps onto the T-DNA. Pools P 6, R 3, C 9 N 3. The insertion site is at the 3' end of the sequence mapping. Genbank ID: KO452208.

Donation Date: 2004-12-01

Donated by:  University of Wisconsin, Madison Patrick Krysan
University of Wisconsin, Madison Richard Amasino
University of Wisconsin, Madison Michael Sussman

Donor Number: WiscDsLox439B09

Part of : Wisconsin DsLox T-DNA lines

Stock type: individual line

Material type: seed

Status Price (£)
Available to order at NASC £10.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-2

Transformation method: Agrobacterium

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code: