NASC Stock Detail Page
NASC ID: N856316
Name: WiscDsLox447F01
ABRC stock number: CS856316
Description: Sequence tagged T-DNA insertion line, generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox; Basta herbicide was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually. This line can be utilized as in Ds Launchpad experiments; the T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line; selection of resulting transposants is by Basta and hygromycin; LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line. Associated Polymorphisms: Wiscseq_DsLox447F01.0 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G28790.1 (at chr 2 pos 12355599 (C/12355599-12355681) on the TAIR10). The sequences' 1-83 bps mapped on the genome, 82-100 bps onto the T-DNA. Pools P 6, R 8, C 3 N 5. The insertion site is at the 3' end of the sequence mapping. Genbank ID: KO452222. Wiscseq_DsLox447F01.1 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G47380.1 (chr 1 pos 17376419 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG805686. Wiscseq_DsLox447F01.2 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G71170.1-UTR3 and AT1G71180.1 (chr 1 pos 26832274 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG805685. Wiscseq_DsLox447F01.3 : Genbank Annotation 2015-02-08 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G61450.2 (chr 3 pos 22738369 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KG805687.
Donation Date: 2004-12-01
| Donated by: | University of Wisconsin, Madison Patrick Krysan University of Wisconsin, Madison Richard Amasino University of Wisconsin, Madison Michael Sussman |
Donor Number: WiscDsLox447F01
Part of : Wisconsin DsLox T-DNA lines
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available to order at NASC | £11.00 |
Germplasm Info
Mutagen: T-DNA Insertion
Background: Col-2
Transformation method: Agrobacterium
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
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