NASC

NASC ID: N879148

Name: SAIL_1277_D09

ABRC stock number: CS879148

Description: T-DNA insertion line, generated by vacuum infiltration of a Columbia (Col) plant with Agrobacterium tumefaciens vector pDAP101, a modified approach of Thermal Asymmetric Interlaced-Polymerase Chain Reaction (TAIL-PCR) was used to amplify DNA fragments flanking the T-DNA left border from this transformed line; BASTA was employed for selection of plants carrying a T-DNA; each T1 transformant has been maintained individually as part of the Syngenta Arabidopsis Insertion Library (SAIL) collection.

Associated Polymorphisms:

SAILseq_1277_D09.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G63250.1 (at chr 3 pos 23372654 (C/23372654-23372704) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 74-95 bps onto the T-DNA. Pools P 12, R 17, C 13 N 18. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO325383.

SAIL_1277_D09

Donation Date: 2006-06-21

Donated by:

Syngenta AG Alberto Martinez Syngenta AG Allen Sessions

Donor Number: SAIL_1277_D09

Part of : SAIL lines from Syngenta

Stock type: individual line

Material type: seed

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: T-DNA Insertion

Background: Col-0(N60000)

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G63250

References

Sessions, A. et al. 2002. A high-throughput Arabidopsis reverse genetics system. The Plant cell 14(12):2985-94.PubMed ID: 12468722.