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NASC Stock Detail Page

NASC ID: N9419

Name: ACT RPS5A #5

ABRC stock number: CS9419

Description: To drive expression of pUAS-controlled genes during early embryogenesis and in dividing tissues.gene product:NPTII and GAL4:VP16.Allele inheritance dominant, kanamycin resistant

Donation Date: 2004-11-15

Donated by:  Leiden University Remko Offringa

Donor Number: ACT RPS5A

Part of : Homozygous lines

Stock type: individual line

Material type: seed

Status Price (£)
Available £7.50

Germplasm Info

Seed type: Promoter Trap

Background: Columbia

Segregation status: homozygous

Growth requirement: none

Segregation: no

Transformation method: Construct pSDM7040 (ACT RPS5A)Right border pNOS-nptII-tNOSAtRPS5A-GAL4:VP16-tNOS Left border Agrobacterium-mediated transformation via floral dip

References

Weijers, D., et al. 2001. Seed development: Early paternal gene activity in Arabidopsis. Nature 414(6865):709-710 .PubMed ID: 11742384.

Weijers, D., et al. 2003. Diphtheria toxin-mediated cell ablation reveals interregional communication during Arabidopsis seed development. Plant Physiology 133(4):1882-1892 .PubMed ID: 14605218.