NASC Stock Detail Page
NASC ID: N9809
Name: F4cca1lhyCCR2:LucW1/34
ABRC stock number: CS9809
Description: Produced by crossing parent CCR2:LUC W1 (deposited with this stock: 9808) to the lhy-21, cca1-11 mutant (stock N9380). Non-segregating LUC+ line selected. Homozygosity of the mutations verified by morphological phenotype and PCR. N9380 was produced by crossing two mutant lines from the Wisconsin T-DNA knockout collection. The CCR2:LUC W1 parent carries a fusion of CCR2 (AtGRP7, At2g21660) upstream region to the LUC+ firefly luciferase reporter gene (from Promega), as described in Doyle et al. (2002) Nature 419: 74-77.
Donation Date: 2007-12-21
| Donated by: | University of Edinburgh Andrew Millar University of Edinburgh Adrian Thomson |
Donor Number: F4cca1lhyCCR2:LucW1/34
Part of : Homozygous lines
Stock type: individual line
Material type: seed
Comment: A set of cca1-11, lhy-21 double mutant lines that were independently transformed with CCR2:LUC were deposited at the same time as this stock: 9810, 9811, 9812. Also see stock 9808 (wild type).
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Seed type: Transgenic
Background: Ws-2
Segregation status: homozygous
Pedigree: Produced by crossing parent CCR2:LUC W1 (deposited with this stock: 9808) to the lhy-21, cca1-11 mutant (stock N9380).
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
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Phenotype
The lhy, cca1 double mutants severely affect flowering time, light responses and circadian rhythms.
References
Locke, J.C. et al. 2006. Experimental validation of a predicted feedback loop in the multi-oscillator clock of Arabidopsis thaliana. Molecular systems biology 2:59.PubMed ID: 17102804.