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NASC Stock Detail Page

NASC ID: N9810

Name: T4cca1lhyCCR2:Luc_233P2E9

ABRC stock number: CS9810

Description: Produced by transforming CCR2:LUC into the lhy-21, cca1-11 mutant (stock N9380). Non-segregating LUC+ line selected. Homozygosity of the mutations verified by morphological phenotype and PCR. N9380 was produced by crossing two mutant lines from the Wisconsin T-DNA knockout collection.The CCR2:LUC reporter is a fusion of CCR2 (AtGRP7, At2g21660) upstream region to the LUC+ firefly luciferase reporter gene (from Promega), as described in Doyle et al. (2002) Nature 419: 74-77.

Donation Date: 2007-12-21

Donated by:  University of Edinburgh Andrew Millar
University of Edinburgh Adrian Thomson

Donor Number: T4cca1lhyCCR2:Luc_233P2E9

Part of : Homozygous lines

Stock type: individual line

Material type: seed

Comment: A cca1-11, lhy-21 double mutant that was crossed to a CCR2:LUC reporter, along with the wild-type parent, were deposited at the same time as this stock: see 9808 (wild type), 9809, 9811, 9812.

Status Price (£)
Available £7.50

Germplasm Info

Seed type: Transgenic

Background: Ws-2

Segregation status: homozygous

Pedigree: Produced by transforming CCR2:LUC into the lhy-21, cca1-11 mutant (stock N9380).

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Allele: Locus: AGI code:
  • cca1-11
  • lhy-21
  • cca1
  • lhy

Phenotype

The lhy, cca1 double mutants severely affect flowering time, light responses and circadian rhythms.

References

Locke, J.C. et al. 2006. Experimental validation of a predicted feedback loop in the multi-oscillator clock of Arabidopsis thaliana. Molecular systems biology 2:59.PubMed ID: 17102804.