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NASC

The European Arabidopsis Stock Centre

NASC Stock Detail Page

NASC ID: N76502

Name: Alonso Crosby and Ecker

ABRC stock number: CS76502

Description: First set of 10,000 T-DNA lines produced by J. Alonso, W. Crosby and J. Ecker; packaged as 100 pools of 100 lines. These insertion lines were generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens. The vector employed was pROK2 (Baulcombe et al., 1986, Nature 321:446). Each T1 transformant was grown individually, and equal quantities of T2 seeds combined to create pools of 10 lines. These pools of seeds were grown at ABRC to generate the T3 seed pools which are the distributed stocks. The parental line (N60000) is included with this stock.

Donation Date: 1999-12-07

Donated by:  The Salk Institute for Biological Studies Joseph R. Ecker
University of Windsor William Crosby
North Carolina State University Jose M. Alonso

Part of : Alonso Crosby and Ecker

Stock type: set (View set contents)

Material type: seed

Status Price (£)
Currently unavailable £107.00

Germplasm Info

Genus: Arabidopsis     Species: thaliana

Mutagen: ema

Background: Col-0(N60000)

Transformation method: These insertion lines were generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens. The vector employed was pROK2 (Baulcombe et al., 1986, Nature 321:446). Each T1 transformant was grown individually, and equal quantities of T2 seeds combined to create pools of 10 lines.

Ecotype data

Location: Columbia

Country: USA

Altitude Min: 1

Altitude Max: 100

Latitude Min: 38.3

Latitude Max: 38.3

Longitude Min: -92.3

Longitude Max: -92.3

Monthly Preciptation Spring Min: 60

Monthly Preciptation Spring Max: 70

Monthly Preciptation Autumn Min: 30

Monthly Preciptation Autumn Max: 40