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NASC

The European Arabidopsis Stock Centre

Isner Collection

Donated by

  • Jean Charles Isner School of Biological Sciences, University of Bristol

Click here to view all 3 of these lines.

Description

This collection is made up of three lines, two plasmid dna stocks (N2105633 & N2105634) and one seed stock (N2105635)

Abstract from paper relating to these stocks

Measurement of cellular cGMP in plant cells and tissues using the endogenous fluorescent reporter FlincG

The cyclic nucleotide cGMP has been shown to play important roles in plant development and responses to abiotic and biotic stress. To date, the techniques that are available to measure cGMP in plants are limited by low spatial and temporal resolution. In addition, tissue destruction is necessary.

To circumvent these drawbacks we have used the δ-FlincG fluorescent protein to create an endogenous cGMP sensor that can report cellular cGMP levels with high resolution in time and space in living plant cells. δ-FlincG in transient and stably expressing cells shows a dissociation constant for cGMP of around 200 nm giving it a dynamic range of around 20–2000 nm. Stimuli that were previously shown to alter cGMP in plant cells (nitric oxide and gibberrellic acid) evoked pronounced fluorescence signals in single cells and in root tissues, providing evidence that δ-FlincG reports changes in cellular cGMP in a physiologically relevant context.

Jean-Charles Isner and Frans J. M. Maathuis

References

  • Isner, J.C. & Maathuis, F.J. 2011. Measurement of cellular cGMP in plant cells and tissues using the endogenous fluorescent reporter FlincG. The Plant Journal 26(2):329-34. PubMed ID: 21223396.