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The European Arabidopsis Stock Centre

LhG4 Mis-expression tags (GMT) - Moore/Clarke/Bevan/Coupland

Donated by

  • Mike Bevan Department of Cell and Developmental Biology, John Innes Centre

Click here to view all 2029 of these lines.

Description

Population characteristics

This population was generated using Ac/Ds transposition to generate enhancer trap patterns of expression for LhG4 as part of a collaboration between Ian Moore, Jonathan Clarke, Mike Bevan and George Coupland (MET/MT).

The system utilises a translational fusion of (a) the transcriptional activation region II from the GAL4 transcription factor and (b) the DNA binding domain of a high affinity lac repressor mutant. Founder constructs were introduced into plants via a T-DNA transformation and a population was generated using 35S-Tpase (which was subsequently removed by counter-selection).

The patterns of expression generated can be detected by GFP/GUS genes under the control of LhG4 dependant (pOp) promoters at an unlinked site. A minimal promoter containing lac repressor, "pOp" has shown to be active in plants in the presence of LhG4, but drives no expression of marker genes in the absence of LhG4.

Pedigree

The GMT stocks were generated in the Landsberg erecta background and are either heterozygous or homozygous for the unique GMT insertion.

They are homozygous resistant to Hygromycin (reporter construct) and heterozygous or homozygous resistant to BAR (for the unique GMT [Ds-MET] insertion). The majority of GMT lines carry a single GMT insertion. A small proportion (2-5%) carry two insertions;

Naming conventions and history

The material was originally described as MT lines - they have subsequently been renamed GMT (Garnet MT) but have also occasionally been called MET lines in the web literature.

Construct topology


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