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NASC

The European Arabidopsis Stock Centre

Wisconsin DsLox T-DNA lines

Donated by

  • Patrick Krysan Department of Horticulture, College of Agricultural and Life Sciences, University of Wisconsin, Madison
  • Richard Amasino Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison
  • Michael Sussman Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin, Madison

Click here to view all 32614 of these lines.

Description

Wisconsin DsLox T-DNA lines

Generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector WiscDs-Lox. Basta herbicide was employed for selection of plants carrying a T-DNA. Each T1 transformant has been maintained individually. They can be utilised as in Ds Launchpad experiments. The T-DNA includes a Ds transposon/launchpad construction so that transposition can be induced by crossing to an Ac line. The selection of resulting transposants is by Basta and hygromycin. LoxP sites are also present so that regions between transposed Ds elements and the empty donor site can be subsequently removed by crossing to a Cre line.

The collection includes more than 30,000 lines.