NASC Stock Detail Page
NASC ID: N16303
Name: Kaleidocell
ABRC stock number: CS16303
Description: Transgenic line tagged with fluorescent proteins. Nuclei, plasma membranes, mitochondria, and plastids are genetically tagged with CFP, GFP, YFP, and RFP respectively. Agrobacterium tumefaciens strain GV3101/pMP90 was transformed with the vectors, pKW102-CY-MODC, pEL103-RecA::DsRed2 , and pEL103-GR respectively. These Agrobacterium strains were then used to transform Arabidopsis thaliana (Columbia accession). Zygosities of each transgenic line were determined by their mortalities on kanamycin selection plates (50 ug/ mL) and by microscopic detections of the fluorescent proteins in their seedlings. Homozygous lines of transgenic plants expressing Gal4-CFP and CoxIV-YFP were crossed to homozygous lines of transgenic plants expressing Cam53BD-GFP. The resulting homozygous lines were further crossed to homozygous transgenic plants expressing RecA-RFP to obtain the Kaleidocell lines. The line that most strongly expressed all transgenes was designated Kaleidocell.17. Kaleidocell.17 was self-pollinated in the following three generations. The line that expressed all transgenes in its all progenies was identified and designated Kaleidocell.17.1.7. The progenies of Kaleidocell.17.1.7 were used as the homozygous of Kaleidocell. The second progenies of Kaleidocell.17.1.7 (designated Kalediocell.17.1.7.7.X) are the distribution stock.
Donation Date: 2008-03-24
| Donated by: | Louisiana State University (LSU) Dexter Reynolds Louisiana State University (LSU) Naohiro Kato |
Donor Number: Kaleidocell.17.1.7.7.X
Part of : Homozygous lines
Stock type: individual line
Material type: seed
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Seed type: Transgenic
Mutagen: ema
Background: Col(Columbia)
Segregation status: homozygous
Phenotype
In fluorescence microscopy, the nuclei, plasma membranes, mitochondria, and plastids fluoresce with cyan, green, yellow, and red respectively.