Home | About NASC | Address/Staff
Ask a question | Help

NASC

Please note that due to the global coronavirus epidemic orders placed with NASC may take longer than usual to reach you

NASC Stock Detail Page

NASC ID: N16303

Name: Kaleidocell

ABRC stock number: CS16303

Description: Transgenic line tagged with fluorescent proteins. Nuclei, plasma membranes, mitochondria, and plastids are genetically tagged with CFP, GFP, YFP, and RFP respectively. Agrobacterium tumefaciens strain GV3101/pMP90 was transformed with the vectors, pKW102-CY-MODC, pEL103-RecA::DsRed2 , and pEL103-GR respectively. These Agrobacterium strains were then used to transform Arabidopsis thaliana (Columbia accession). Zygosities of each transgenic line were determined by their mortalities on kanamycin selection plates (50 ug/ mL) and by microscopic detections of the fluorescent proteins in their seedlings. Homozygous lines of transgenic plants expressing Gal4-CFP and CoxIV-YFP were crossed to homozygous lines of transgenic plants expressing Cam53BD-GFP. The resulting homozygous lines were further crossed to homozygous transgenic plants expressing RecA-RFP to obtain the Kaleidocell lines. The line that most strongly expressed all transgenes was designated Kaleidocell.17. Kaleidocell.17 was self-pollinated in the following three generations. The line that expressed all transgenes in its all progenies was identified and designated Kaleidocell.17.1.7. The progenies of Kaleidocell.17.1.7 were used as the homozygous of Kaleidocell. The second progenies of Kaleidocell.17.1.7 (designated Kalediocell.17.1.7.7.X) are the distribution stock.

Donation Date: 2008-03-24

Donated by:  Louisiana State University (LSU) Dexter Reynolds
Louisiana State University (LSU) Naohiro Kato

Donor Number: Kaleidocell.17.1.7.7.X

Part of : Homozygous lines

Stock type: individual line

Material type: seed

Status Price (£)
Available £7.50

Germplasm Info

Seed type: Transgenic

Mutagen: ema

Background: Col(Columbia)

Segregation status: homozygous

Phenotype

In fluorescence microscopy, the nuclei, plasma membranes, mitochondria, and plastids fluoresce with cyan, green, yellow, and red respectively.