NASC Stock Detail Page
NASC ID: N532720
Name: SALK_032720
ABRC stock number: SALK_032720
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_032720.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G35930.1 (at chr 4 pos 17019929 (W/17019882-17019929) on the TAIR10). The sequences' 1-48 bps mapped on the genome, 56-79 bps onto the T-DNA. Pools P 2, R 5, C 8 N 9. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO354369. SALKseq_032720.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G30240.1 (at chr 1 pos 10642325 (C/10642325-10642376) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 2, R 5, C 8 N 9. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO344332. SALKseq_032720.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G06290.1=UTR3 and AT5G06300.1=UTR3 (at chr 5 pos 1921504 (W/1921453-1921504) on the TAIR10). The sequences' 1-52 bps mapped on the genome, 50-79 bps onto the T-DNA. Pools P 2, R 5, C 8 N 9. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO357696. SALKseq_032720.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G50650.1 and AT1G50660.1=P355 (at chr 1 pos 18769198 (C/18769198-18769263) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 2, R 5, C 8 N 9. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO347676. SALKseq_032720.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G34610.1=P355 and AT4G34620.1=UTR3 (at chr 4 pos 16534165 (W/16534118-16534165) on the TAIR10). The sequences' 1-48 bps mapped on the genome, 50-76 bps onto the T-DNA. Pools P 2, R 5, C 8 N 9. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO351027.
Donation Date: 2002-01-02
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_032720
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N665704 (SALK_032720C).
Status | Price (£) |
---|---|
Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
---|---|---|---|
|
|
|
Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At4g35930 T19K4.60 Exon
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.