NASC

NASC ID: N532887

Name: SALK_032887

ABRC stock number: SALK_032887

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_032887.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G21140.1=UTR3 and AT1G21150.1=UTR3 (at chr 1 pos 7406147 (W/7406097-7406147) on the TAIR10). The sequences' 1-51 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 2, R 5, C 3 N 1. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO397882.

SALKseq_032887.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G01350.1 (at chr 2 pos 165795 (W/165752-165795) on the TAIR10). The sequences' 1-44 bps mapped on the genome, 51-77 bps onto the T-DNA. Pools P 2, R 5, C 3 N 1. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO401225.

SALKseq_032887.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G52820.1=P355 and AT3G52830.1 (at chr 3 pos 19577598 (W/19577542-19577598) on the TAIR10). The sequences' 1-57 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 2, R 5, C 3 N 1. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO404558.

SALKseq_032887.5 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G13266.1=P355 and AT4G13261.1 (around chr 4 pos 7708317 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO414595.

SALKseq_032887.7 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G35405.1 and AT5G35407=P355 (at chr 5 pos 13605079 (W/13605020-13605079) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 2, R 5, C 3 N 1. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO411241.

Donation Date: 2001-01-12

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_032887

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N665706 (SALK_032887C).

Status	Price (£)
Available to order at NASC	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G21140 AT1G21150 AT2G01350 AT3G52820 AT3G52830 AT4G13261 AT4G13266 At4g18520 AT5G35405 AT5G35407

EMBL accession:

ED580040

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.