NASC

NASC ID: N533618

Name: SALK_033618

ABRC stock number: SALK_033618

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_033618.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G61660.1 (at chr 1 pos 22754949 (W/22754882-22754949) on the TAIR10). The sequences' 1-68 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 9, R 1, C 2 N 3. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO394575.

SALKseq_033618.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G70010.1 (at chr 1 pos 26369703 (W/26369650-26369703) on the TAIR10). The sequences' 1-54 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 9, R 1, C 2 N 3. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO397914.

SALKseq_033618.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G12470.1 (at chr 5 pos 4046098 (C/4046098-4046162) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 9, R 1, C 2 N 3. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO401256.

Donation Date: 2001-01-12

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_033618

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N653129 (SALK_033618C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G70010 AT5G12470 At1g61660

EMBL accession:

ED580519

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At1g61660

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.