NASC Stock Detail Page
NASC ID: N534154
Name: SALK_034154
ABRC stock number: SALK_034154
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_034154.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G60318.1 (at chr 3 pos 22291761 (W/22291707-22291761) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 7, R 1, C 4 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO411299. SALKseq_034154.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G36200.1 (around chr 1 pos 13580918 on the TAIR10). The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO414657. SALKseq_034154.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G25020.1 and AT3G25030.1=P355 (at chr 3 pos 9121789 (W/9121742-9121789) on the TAIR10). The sequences' 1-47 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 7, R 1, C 4 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO341041. SALKseq_034154.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G69180.1=P355 and AT1G69190.1=UTR3 (at chr 1 pos 26011708 (W/26011672-26011708) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 57-79 bps onto the T-DNA. Pools P 7, R 1, C 4 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO404618. SALKseq_034154.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G23560.1=P355 and AT1G23570.2=UTR3 (at chr 1 pos 8354678 (C/8354678-8354720) on the TAIR10). The sequences' 1-43 bps mapped on the genome, 46-75 bps onto the T-DNA. Pools P 7, R 1, C 4 N 2. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO407952.
Donation Date: 2001-01-12
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_034154
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_034154C.
Status | Price (£) |
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Available to order at NASC | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.