NASC Stock Detail Page
NASC ID: N537261
Name: SALK_037261
ABRC stock number: SALK_037261
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_037261.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G32180.1 and AT1G32190.1=UTR3 (at chr 1 pos 11590093 (W/11590033-11590093) on the TAIR10). The sequences' 1-61 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 10, R 6, C 10 N 10. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO388042. SALKseq_037261.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G01610.1=P355 and AT1G01620.1=UTR3 (at chr 1 pos 224778 (W/224724-224778) on the TAIR10). The sequences' 1-55 bps mapped on the genome, 51-79 bps onto the T-DNA. Pools P 10, R 6, C 10 N 10. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO391385. SALKseq_037261.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT1G11840.2 (at chr 1 pos 3995375 (C/3995375-3995436) on the TAIR10). The sequences' 1-62 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 10, R 6, C 10 N 10. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO394739. SALKseq_037261.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G20860.1 (at chr 3 pos 7308429 (C/7308429-7308491) on the TAIR10). The sequences' 1-63 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 10, R 6, C 10 N 10. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO398079. SALKseq_037261.4 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G20550.1=P355 and AT5G20560.1 (at chr 5 pos 6954378 (C/6954378-6954435) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 59-79 bps onto the T-DNA. Pools P 10, R 6, C 10 N 10. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO401420.
Donation Date: 2002-01-01
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_037261
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_037261C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.