NASC

NASC ID: N539148

Name: SALK_039148

ABRC stock number: SALK_039148

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_039148.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT1G12672.2=UTR3 and AT1G12680.1=UTR3 (at chr 1 pos 4319155 (W/4319092-4319155) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 61-79 bps onto the T-DNA. Pools P 10, R 5, C 7 N 4. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO358016.

SALKseq_039148.001 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G15330.1 (at chr 5 pos 4980465 (C/4980465-4980543) on the TAIR10). The sequences' 1-79 bps mapped on the genome. Pools P 10, R 5, C 7 N 4. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO364726.

SALKseq_039148.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G46040.1 (at chr 5 pos 18672020 (W/18671957-18672020) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 10, R 5, C 7 N 4. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO361365.

Donation Date: 2002-01-01

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_039148

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_039148C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G12672 AT1G12680 AT5G46040 At5g15330

EMBL accession:

BH746312

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g15330

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.