NASC Stock Detail Page
NASC ID: N543961
Name: SALK_043961
ABRC stock number: SALK_043961
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_043961.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT3G11960.1 (at chr 3 pos 3792333 (W/3792269-3792333) on the TAIR10). The sequences' 1-65 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 14, R 15, C 15 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO381672. SALKseq_043961.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G80100.1 (at chr 1 pos 30134362 (C/30134362-30134406) on the TAIR10). The sequences' 1-45 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 14, R 15, C 15 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO371626. SALKseq_043961.101 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G58630.1 (at chr 5 pos 23697667 (W/23697621-23697667) on the TAIR10). The sequences' 1-47 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 14, R 15, C 15 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc KO385022. SALKseq_043961.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G24120.1 (at chr 1 pos 8530096 (C/8530096-8530148) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 51-77 bps onto the T-DNA. Pools P 14, R 15, C 15 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO374981. SALKseq_043961.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G24620.1 (at chr 2 pos 10463386 (W/10463350-10463386) on the TAIR10). The sequences' 1-37 bps mapped on the genome, 40-62 bps onto the T-DNA. Pools P 14, R 15, C 15 N 14. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO378326.
Donation Date: 2002-01-01
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_043961
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock is only available on request as a homozygous/confirmed stock has replaced it. The replacement line is SALK_043961C.
Status | Price (£) |
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Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At3g11960
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.