NASC Stock Detail Page
NASC ID: N545849
Name: SALK_045849
ABRC stock number: SALK_045849
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_045849.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G32047.1 and AT3G32050.1 (at chr 3 pos 13064753 (W/13064709-13064753) on the TAIR10). The sequences' 1-45 bps mapped on the genome, 62-79 bps onto the T-DNA. Pools P 13, R 20, C 13 N 15. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO348313. SALKseq_045849.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G63560.1 (at chr 5 pos 25449330 (W/25449273-25449330) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 60-79 bps onto the T-DNA. Pools P 13, R 20, C 13 N 15. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO351668.
Donation Date: 2002-01-02
| Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_045849
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: Homozygous/confirmed line: SALK_045849C.
| Status | Price (£) |
|---|---|
| Available | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
| Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At4g27750 T29A15.240 Exon
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.