NASC

NASC ID: N547814

Name: SALK_047814

ABRC stock number: SALK_047814

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

Associated Polymorphisms:

SALKseq_047814.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G77280.1 (at chr 1 pos 29034941 (W/29034904-29034941) on the TAIR10). The sequences' 1-38 bps mapped on the genome, 55-79 bps onto the T-DNA. Pools P 19, R 14, C 13 N 12. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO345067.

SALKseq_047814.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT3G59430.2 (at chr 3 pos 21967372 (C/21967372-21967404) on the TAIR10). The sequences' 1-33 bps mapped on the genome, 28-57 bps onto the T-DNA. Pools P 19, R 14, C 13 N 12. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO348414.

SALKseq_047814.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G59790.1=UTR3 and AT5G59800.1=UTR3 (at chr 5 pos 24094367 (C/24094367-24094423) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 58-79 bps onto the T-DNA. Pools P 19, R 14, C 13 N 12. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO351769.

Donation Date: 2002-01-02

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_047814

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N662373 (SALK_047814C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT3G59430 AT5G59790 AT5G59800 At1g77280

EMBL accession:

BH749403

Phenotype

Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation - At1g77280 T14N5.13 Intron

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.