NASC

NASC ID: N549127

Name: SALK_049127

ABRC stock number: SALK_049127

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

March 2002 TAIR gene annotation: MDA7.16 L300-5' Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g56100.

Associated Polymorphisms:

SALKseq_049127.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G56100.1 (at chr 5 pos 22716970 (W/22716905-22716970) on the TAIR10). The sequences' 1-66 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 23, R 27, C 22 N 25. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO402023.

SALKseq_049127.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G39800.1=P355 and AT2G39805.1=UTR3 (at chr 2 pos 16607992 (C/16607992-16608069) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 23, R 27, C 22 N 25. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO395336.

SALKseq_049127.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G47150.1 and AT5G47160.1 (at chr 5 pos 19152087 (W/19152023-19152087) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 58-78 bps onto the T-DNA. Pools P 23, R 27, C 22 N 25. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO398675.

Donation Date: 2002-03-21

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_049127

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_049127C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G39800 AT2G39805 AT5G47150 AT5G47160 At5g56100

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.