NASC

NASC ID: N549567

Name: SALK_049567

ABRC stock number: SALK_049567

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

April 2002 TAIR gene annotation: MFB13.8 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At5g61440.

Associated Polymorphisms:

SALKseq_049567.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G61440.1 (at chr 5 pos 24708521 (W/24708469-24708521) on the TAIR10). The sequences' 1-53 bps mapped on the genome, 48-76 bps onto the T-DNA. Pools P 22, R 28, C 27 N 27. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO388670.

SALKseq_049567.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G11200.1 (at chr 4 pos 6829583 (W/6829519-6829583) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 61-78 bps onto the T-DNA. Pools P 22, R 28, C 27 N 27. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO385323.

Donation Date: 2002-04-30

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_049567

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: Homozygous/confirmed line: SALK_049567C.

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT4G11200 At5g61440

EMBL accession:

ED588868

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.