NASC

NASC ID: N552858

Name: SALK_052858

ABRC stock number: SALK_052858

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.

March 2002 TAIR gene annotation: T30B22.18 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At2g47520.

Associated Polymorphisms:

SALKseq_052858.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT4G29930.1 (at chr 4 pos 14643991 (W/14643959-14643991) on the TAIR10). The sequences' 1-33 bps mapped on the genome, 43-71 bps onto the T-DNA. Pools P 22, R 27, C 29 N 27. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO405494.

SALKseq_052858.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT5G07920.1 (at chr 5 pos 2529418 (C/2529418-2529463) on the TAIR10). The sequences' 2-48 bps mapped on the genome, 55-78 bps onto the T-DNA. Pools P 22, R 27, C 29 N 27. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO408831.

SALKseq_052858.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT5G12260.1 (at chr 5 pos 3968028 (W/3967965-3968028) on the TAIR10). The sequences' 1-64 bps mapped on the genome, 60-78 bps onto the T-DNA. Pools P 22, R 27, C 29 N 27. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO412181.

SALKseq_052858.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT5G22780.1=P355 and AT5G22790.1=UTR3 (at chr 5 pos 7598785 (W/7598708-7598785) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 22, R 27, C 29 N 27. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO341927.

Donation Date: 2002-03-21

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_052858

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N666041 (SALK_052858C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT4G29930 AT5G07920 AT5G12260 AT5G22780 AT5G22790 At2g47520

EMBL accession:

BH789946

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.