NASC

NASC ID: N553593

Name: SALK_053593

ABRC stock number: SALK_053593

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.March 2002 TAIR gene annotation: F24L7.12 Exon Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At2g32740

Associated Polymorphisms:

SALKseq_053593.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G32740.1 (at chr 2 pos 13887002 (W/13886955-13887002) on the TAIR10). The sequences' 1-48 bps mapped on the genome, 75-95 bps onto the T-DNA. Pools P 29, R 25, C 30 N 24. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO385484.

SALKseq_053593.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G10150.1 (at chr 2 pos 3844413 (W/3844369-3844413) on the TAIR10). The sequences' 1-45 bps mapped on the genome, 57-78 bps onto the T-DNA. Pools P 29, R 25, C 30 N 24. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO388831.

SALKseq_053593.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G15115.1=P355 and AT3G15120.1=UTR3 (at chr 3 pos 5088198 (W/5088120-5088198) on the TAIR10). The sequences' 1-79 bps mapped on the genome, 78-95 bps onto the T-DNA. Pools P 29, R 25, C 30 N 24. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO392164.

SALKseq_053593.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the UTR 5' of AT3G02700.1 (at chr 3 pos 582871 (W/582812-582871) on the TAIR10). The sequences' 1-60 bps mapped on the genome, 78-95 bps onto the T-DNA. Pools P 29, R 25, C 30 N 24. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO395519.

SALKseq_053593.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT3G22142.1 and AT3G22150.1=P355 (at chr 3 pos 7808320 (C/7808320-7808377) on the TAIR10). The sequences' 1-58 bps mapped on the genome, 76-95 bps onto the T-DNA. Pools P 29, R 25, C 30 N 24. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO398858.

Donation Date: 2002-06-26

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_053593

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N659399 (SALK_053593C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT2G10150 AT3G02700 AT3G15115 AT3G15120 AT3G22142 AT3G22150 At2g32740

EMBL accession:

BH756380

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.