NASC Stock Detail Page
NASC ID: N554324
Name: SALK_054324
ABRC stock number: SALK_054324
Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material. Associated Polymorphisms: SALKseq_054324.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G10260.1 (at chr 1 pos 3360899 (C/3360899-3360976) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 29, R 24, C 27 N 26. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO375479. SALKseq_054324.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT2G03740.1 (at chr 2 pos 1143171 (W/1143130-1143171) on the TAIR10). The sequences' 1-41 bps mapped on the genome, 72-95 bps onto the T-DNA. Pools: P 29, R 24, C 27 N 26. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO378825. SALKseq_054324.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT2G02180.1=UTR3 and AT2G02190.1=P355 (at chr 2 pos 564865 (C/564865-564959) on the TAIR10). The sequences' 1-95 bps mapped on the genome, 3-20 bps onto the T-DNA. Pools P 29, R 24, C 27 N 26. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO382170. SALKseq_054324.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the Intergenic between AT4G34065 and AT4G34070.1=UTR3 (at chr 4 pos 16322468 (W/16322393-16322468) on the TAIR10). The sequences' 1-76 bps mapped on the genome, 73-95 bps onto the T-DNA. Pools P 29, R 24, C 27 N 26. The insertion site is at the 3' end of the sequence mapping.. Genbank Acc. KO385523.
Donation Date: 2002-03-21
Donated by: | The Salk Institute for Biological Studies Joseph R. Ecker |
Donor Number: SALK_054324
Part of : SALK lines
Stock type: individual line
Material type: seed
Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N654653 (SALK_054324C).
Status | Price (£) |
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Available to order at NASC | £11.00 |
Germplasm Info
Mutagen: ema
Background: Col-0 (N60000)
Segregation status: segregating
Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
Associated Polymorphisms
When available, select a locus to display it at the AIP or view the EMBL record at the EBI.
Gene: | Allele: | Locus: | AGI code: |
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Phenotype
Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2
References
Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.