NASC

NASC ID: N554337

Name: SALK_054337

ABRC stock number: SALK_054337

Description: Sequence-indexed T-DNA insertion line generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2; kanamycin was employed for selection of plants carrying a T-DNA (please note that in many lines, the kan resistance trait has been cosuppressed, so that insertion plants may not be kanamycin resistant); each T1 transformant has been maintained individually at SALK; the DNA sequence of each T-DNA flanking region was generated from seedlings grown from the same sample of seeds as that provided for distribution (T3). NOTE: kanamycin resistance gene may be silenced; PCR- or hybridization-based segregation analysis is required to confirm presence of insertion; may be segregating for phenotypes that are not linked to the insertion; may have additional insertions potentially segregating. Please cite the Alonso et al. reference linked to this stock and acknowledge NASC/ABRC for distributing the seeds in all presentations and publications utilizing this material.March 2002 TAIR gene annotation: F22G10.31 Intron Single, segregating flank-tagged T3-generation T-DNA lines generated by vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2 : gene annotation At1g53730

Associated Polymorphisms:

SALKseq_054337.0 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G53730.1 (at chr 1 pos 20064536 (C/20064536-20064591) on the TAIR10). The sequences' 1-56 bps mapped on the genome, 52-73 bps onto the T-DNA. Pools P 26, R 29, C 25 N 21. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO352059.

SALKseq_054337.1 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT1G58220.1 (at chr 1 pos 21563094 (W/21563017-21563094) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 26, R 29, C 25 N 21. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO355393.

SALKseq_054337.2 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT1G19392.1 (at chr 1 pos 6709160 (W/6709084-6709160) on the TAIR10). The sequences' 1-77 bps mapped on the genome. Pools P 26, R 29, C 25 N 21. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO358732.

SALKseq_054337.3 : Genbank Annotation 2015-02-18 - This genomic-terminal sequence of a TDNA insertion region lies within the Promoter 500bps of AT2G43520.1 (at chr 2 pos 18068333 (W/18068256-18068333) on the TAIR10). The sequences' 1-78 bps mapped on the genome. Pools P 26, R 29, C 25 N 21. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO362076.

SALKseq_054337.4 : Genbank Annotation 2015-02-18 -This genomic-terminal sequence of a TDNA insertion region lies within the CDS of AT4G16310.1 (at chr 4 pos 9223129 (W/9223062-9223129) on the TAIR10). The sequences' 1-69 bps mapped on the genome, 52-79 bps onto the T-DNA. Pools P 26, R 29, C 25 N 21. The insertion site is at the 3' end of the sequence mapping. Genbank Acc. KO365427.

Donation Date: 2002-03-21

Donated by:

The Salk Institute for Biological Studies Joseph R. Ecker

Donor Number: SALK_054337

Part of : SALK lines

Stock type: individual line

Material type: seed

Comment: This segregating stock has been superseded by a homozygous/confirmed stock which can be ordered as an alternative. The replacement line is N653212 (SALK_054337C).

Status	Price (£)
Available	£11.00

Germplasm Info

Mutagen: ema

Background: Col-0 (N60000)

Segregation status: segregating

Transformation method: Vacuum infiltration of Columbia (Col) plants with Agrobacterium tumefaciens vector pROK2

Associated Polymorphisms

When available, select a locus to display it at the AIP or view the EMBL record at the EBI.

Gene:	Allele:	Locus:	AGI code:
			AT1G19392 AT1G58220 AT2G43520 AT4G16310 At1g53730

EMBL accession:

BH790018

References

Alonso, J.M. et al. 2003. Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301(5633):653-7.PubMed ID: 12893945.